신경모세포종 세포배양에서 글루탐산염에 의해 유발된 흥분성세포독성에 대한 Indirubin?3’一monoxime의 보호 효과
Indirubin-3-monoxime inhibits excitotoxicity provoked by glutamate on neuroblastoma cell culture
한승연, 정보현, 이희수, 유기연,
소속 상세정보
한승연 ( Han Seung-Yun ) - 건양대학교 의과대학 해부학교실
정보현 ( Jung Bo-Hyun ) - 강릉원주대학교 치과대학 구강해부학교실
이희수 ( Lee Hee-Su ) - 강릉원주대학교 치과대학 구강해부학교실
유기연 ( Yoo Ki-Yeon ) - 강릉원주대학교 치과대학 구강해부학교실
KMID : 0355820140350010011
Abstract
Indirubin is the active component of Danggui Luhui Wan, a Chinese traditionally medicine. Recently, pharmaceutical researches have focused on its inhibitory effect of cyclin-dependent kinases (Cdks) and glycogen synthase kinase~3beta (GSK3"). Indirubin-3’-monoxime (IMX), a synthetic derivative by indirubin, is commonly used to in vitro studies for indirubin. In the present study, we investigated the inhibitory effect of IMX on glutamate-induced excitotoxicity in human neuroblastoma SH-SY5Y cells. Glutamate-induced cytotoxicity was verified characterized by decreased cell viability. However, treatment of IMX attenuated the glutamate-induced cell death in a dose-dependent manner. This result suggests that IMX may inhibit the neuronal death in various neurodegenerative diseases including stroke.
키워드
indirubin-3'-monoxime; excitotoxicity; glutamate; SH-SY5Y
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